![]() ![]() Choosing a random time point (X) post-treatment may result in no data and/or artefactual data that do not reflect the true response of the tested targets. (B) Time dependence on transcription of targets A, B, and C post-treatment. Wells: total number of microtiter plate wells required for qPCR exclusive of optimization experiments for primer validation (see Figure 4). Targets: genes interrogated for differential expression or abundance including chosen reference genes. ![]() Technical replicate (Tech reps): number of repeats per Bio rep (i.e., same cDNA/gDNA sample pipetted into multiple wells). Biological replicate (Bio reps): number of independent biological specimens from which cDNA/gDNA is produced (i.e., individual samples). Time point (T) (i.e., 0 hour (T 0) and 1 hour (T 1) post-treatment). (A) Biological group (BG): tested experimental conditions (i.e., control (BG 1) vs treatment (BG 2). Figure 1 Planning and Scoping a Quantitative PCR (qPCR) Experiment. ![]()
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